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產(chǎn)品目錄

普諾賽NCI-H146 [H146] (人小細(xì)胞肺癌細(xì)胞)

品名: 普諾賽NCI-H146 [H146] (人小細(xì)胞肺癌細(xì)胞)
型號(hào): CL-0693
  • 產(chǎn)品詳情
  • 規(guī)格參數(shù)

產(chǎn)品信息

名稱NCI-H146 [H146] (人小細(xì)胞肺癌細(xì)胞) (STR鑒定正確)
別稱H146; H-146; NCIH146
背景描述Derived from the pleural fluid of a patient with small cell lung cancer; the cells express elevated levels of the 4 biochemical markers characteristic of SCLC: neuron-specific enolase, the brain isoenzyme of creatine kinase, L-dopa decarboxylase and bombesin-like immunoreactivity; the cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein; the cells express relatively high amounts of c-myc mRNA, but no amplification of the gene is detected.
種屬人類
年齡(性別)男性,59歲
組織來源
生長特性懸浮細(xì)胞
細(xì)胞形態(tài)淋巴母細(xì)胞樣
細(xì)胞類型腫瘤細(xì)胞
腫瘤類型肺癌細(xì)胞
生長培養(yǎng)基RPMI-164010% FBS1% P/S
推薦傳代比例1:2-1:3
推薦換液頻率2~3次/周
凍存條件凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件氣相:空氣,95%;CO2,5%溫度:37℃
基因表達(dá)情況vasopressin negative; oxytocin negative; gastrin releasing peptide negative; vimentin positive by immunostaining; keratin positive by immunostaining; neurofilament triplet protein negative; c-myb mRNA; v-fms mRNA; Ha-ras mRNA; Ki-ras mRNA; N-ras mRNA; c-raf 1 mRNA; myc+; myb+; raf+; ras+; fms+; fes -
注意事項(xiàng)該細(xì)胞為懸浮細(xì)胞,請(qǐng)注意離心收集細(xì)胞懸液;請(qǐng)勿直接倒掉細(xì)胞培養(yǎng)液。
保藏機(jī)構(gòu)ATCC; HTB-173
STR鑒定
  • STR位點(diǎn)信息

    AmelogeninX
    CSF1PO11
    D2S133819
    D3S135811
    D5S81812
    D7S8209,10
    D8S117912,14
    D13S31712
    D16S53911
    D18S5115,17
    D19S43313,14
    D21S1130
    FGA23
    PentaD13
    PentaE12
    TH016,9.3
    TPOX8,11
    vWA14,16
    D6S104311,19
    D12S39118.3,25
    D2S44110,14


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