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產(chǎn)品目錄

普諾賽MT-4 (人T細(xì)胞白血病細(xì)胞)

品名: 普諾賽MT-4 (人T細(xì)胞白血病細(xì)胞)
型號(hào): CL-0655
  • 產(chǎn)品詳情
  • 規(guī)格參數(shù)

產(chǎn)品信息

名稱MT-4 (人T細(xì)胞白血病細(xì)胞) (STR鑒定正確)(僅提供凍存管)
背景描述Derived from a 50 year old Japanese male with generalised lymphadenopathy and heptosplenomegaly (Adult T-cell Leukaemia) by co-culture of his peripheral leukocytes with male umbilical cord lymphocytes. The cells carry HTLV-1 and support the growth of HIV. The cells should be handled under laboratory containment level 3 conditions.
種屬
組織來(lái)源淋巴細(xì)胞
生長(zhǎng)特性懸浮細(xì)胞
細(xì)胞形態(tài)淋巴細(xì)胞樣
細(xì)胞類型腫瘤細(xì)胞
腫瘤類型淋巴瘤細(xì)胞
生長(zhǎng)培養(yǎng)基RPMI-164010% FBS1% P/S
推薦傳代比例3×10^5-5×10^5cells/mL
推薦換液頻率2~3次/周
凍存條件凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件氣相:空氣,95%;CO2,5%
注意事項(xiàng)該細(xì)胞為懸浮細(xì)胞,請(qǐng)注意離心收集細(xì)胞懸液;請(qǐng)勿直接倒掉細(xì)胞培養(yǎng)液。
STR鑒定
  • STR位點(diǎn)信息

    AmelogeninX,Y
    CSF1PO11,12
    D2S133818,23
    D3S135817
    D5S81810,11
    D7S8208,10
    D8S117910,15
    D13S31712
    D16S5399,12
    D18S5113
    D19S43314.2,15.2
    D21S1128,30.3
    FGA23
    PentaD10,13
    PentaE5,15
    TH017
    TPOX11
    vWA17,18
    D6S104312
    D12S39118,21
    D2S44110


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