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產(chǎn)品目錄

普諾賽SNB-19 (人膠質(zhì)瘤細(xì)胞)

品名: 普諾賽SNB-19 (人膠質(zhì)瘤細(xì)胞)
型號(hào): CL-0582
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產(chǎn)品信息

名稱SNB-19 (人膠質(zhì)瘤細(xì)胞) (U-251 MG污染細(xì)胞系,暫不供應(yīng))
別稱SNB.19; SNB19
背景描述Established from the surgical resection of a left parieto-occipital glioblastoma from a 47-year-old man in 1980; cells were described in the literature to secrete plasminogen activator, to be clonogenic in soft agar and to be tumorigenic in nude mice; DNA fingerprinting showed unequivocally that SNB-19 is a subclone of the astrocytoma cell line U-251 MG)。(STR檢測(cè)位點(diǎn)同U-251MG)
種屬
年齡(性別)男;75歲
組織來源星形膠質(zhì)瘤(U-251MG)
生長特性貼壁細(xì)胞
細(xì)胞形態(tài)成纖維細(xì)胞樣
細(xì)胞類型腫瘤細(xì)胞
腫瘤類型膠質(zhì)瘤細(xì)胞
生物安全等級(jí)1
生長培養(yǎng)基DMEM10% FBS1% P/S
推薦傳代比例1:4-1:6
推薦換液頻率2~3次/周
倍增時(shí)間~24-36 hours
凍存條件凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件氣相:空氣,95%;CO2,5%溫度:37℃
保藏機(jī)構(gòu)DSMZ; ACC-325


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